diallel analysis of callus induction and plant regeneration in rice anther culture

نویسندگان

مریم حسینی

عضو هیات علمی موسسه تحقیقات برنج کشور، رشت علی اکبر عبادی

عضو هیات علمی موسسه تحقیقات برنج کشور، رشت حمید شفیعی ثابت

کارشناس موسسه تحقیقات برنج کشور، رشت

چکیده

low efficiency of callus induction and green-plant regeneration have limited the application of anther culture in breeding programs. this study was conducted to determine the inheritance of callus induction and plant regeneration in rice (oryza sativa) anther culture. ten genotypes drived from a five-parent diallel mating set contain khazar, sepidrood, hashemi, alikazemi and nematt were evaluated for their callus induction and plant regeneration abilities. analysis of variance for gca and sca showed significant effect for all traits. the highest gca in green and total regeneration were in khazar and hashemi, so these parents were good for production homozy gose lines. narrowsense heritability were low in this three traits. so non-additive effets were high in these traits. analysis of hymen method showed these conculution too. the genes with increasing effects on green plant and total regeneration percentage were dominant. therefore, we will observed the highest regeneration in f1 family.

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